fitc anti mouse cd31 antibody  (Miltenyi Biotec)

Journal: Molecular Therapy Advances

Article Title: Topical delivery of a PPARγ agonist enables adipogenic repair of irradiated skin

doi: 10.1016/j.omta.2026.201732

Figure Lengend Snippet: Topical rosiglitazone enhances wound healing in irradiated skin (A) Schematic of in vivo radiation and wound healing design. (B) Representative images of wound healing progression in irradiated mice treated with TC (top) or TR (bottom). (C) Kaplan-Meier curve showing time to complete wound closure in TC- and TR-treated irradiated mice. (D) Representative immunofluorescence (IF) images of CD31 expression (20×; scale bars, 100 μm). (E) Quantification of CD31 IF intensity (arbitrary units, a.u.). (F) Representative Picrosirius red-stained images under polarized light, showing collagen fiber maturity and organization (20×; scale bars, 100μm). (G) Quantification of collagen composition (percent area) for type 3 (green/yellow) and type 1 (orange/red) collagen. n = 7 mice/group for wound healing analyses; n = 6–7 mice/group for IF and histology. For IF and Picrosirius: one section/mouse, four high-power fields (hpf)/section (4 measurements/mouse). Statistical analysis: log-rank test (C), unpaired Student’s t test (E, G). ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: For IF imaging using fluorescein isothiocyanate (FITC)-conjugated CD31, cryosectioned tissues were incubated overnight at a concentration of 1:100 (Invitrogen, catalog #2373735).

Techniques: Irradiation, In Vivo, Immunofluorescence, Expressing, Staining

Journal: Pharmaceutics

Article Title: Taming the Tumor Stroma: A Two-Stage Targeted Nanocapsule for Potent Deep Chemo-Immunotherapy in Triple-Negative Breast Cancer

doi: 10.3390/pharmaceutics18020184

Figure Lengend Snippet: NPC-ABS/FDS mediated deep chemotherapy and induced immune activation. ( A , B ) Confocal microscopy images in 3D tumor spheres after treated with different groups (a. NPC-FCS, b. NPC-FCS+NPC-AEAA-SLN, c. NPC-FCS+BAE-Sol, d. NPC-FCS+ABS, and e. NPC-ABS/FCS. Z-axis scan spacing = 10 μm. BAE = 2 μg/mL in A and BAE = 5 μg/mL in B, respectively; the magnification was 10×). ( C ) Representative photomicrographs of tumor biopsies treated with different groups (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS). Tumor blood vessels were labeled with FITC-CD31; the magnification was 20×). Relative expression quantity of immune-associated cells in mouse tumors after administration of different preparations of ( D ) CD4 + T cells, ( E ) CD8 + T cells, and ( F ) Tregs (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS. Mean ± SD, n = 3. ** p < 0.01, *** p < 0.001. Blue color represented positive cells; the magnification was 10×).

Article Snippet: FITC Plus Anti-Mouse CD31 was obtained from Proteintech Group, Inc. (Chicago, IL, USA).

Techniques: Activation Assay, Confocal Microscopy, Saline, Labeling, Expressing